Ellular response to glucose starvation cellular response to heat cellular response to ethanol cellular response to nitrosative pressure cellular response to freezing cellular hypotonic response cellular hyperosmotic salinity response cellular response to alkalinity cellular response to acidity cellular response to hydrostatic stress cellular response to arsenic-containing substance cellular response to cold cellular response to hydrogen peroxide good regulation of transcription from RNA polymerase II promoter in response to pressure age-dependent response to oxidative pressure involved in chronological cell aging protein tyrosine kinase activity good regulation of cytoplasmic mRNA processing physique assembly nucleic acid binding protein phosphatase form 1 complex translational initiation translation initiation factor activity helicase activity protein phosphatase regulator activity methylated histone residue binding regulation of ER to Golgi vesicle-mediated transport ribophagy regulation of retrograde vesicle-mediated transport, Golgi to ER ascospore-type prospore assembly 1-phosphatidylinositol-4-phosphate 5-kinase activity nucleosome positioning phosphatidylinositol phosphate kinase activity phosphatidylinositol metabolic procedure adverse regulation of histone H3-K9 acetylation negative regulation of DNA-dependent DNA replication negative regulation of histone H3-K14 acetylation chromatin DNA binding nucleolar chromatin termination of RNA polymerase II transcription termination of RNA polymerase I transcription protein serine/threonine/tyrosine kinase activity regulation of transcriptional commence internet site selection at RNA polymerase II promoterE15 7.2212021-40-2 structure 5 0 -7.3-(Dibenzylamino)propan-1-ol custom synthesis five -15 15 7.five 0 -7.five -15 difference (P-value 0.05) 15 7.5 0 -7.five -15 15 7.five 0 -7.5 -15 15 7.five 0 -7.five -15 15 7.5 0 -7.5 -ClusterClusterRelative phosphorylation changeClusterClusterClusterClusterClusterClusterClusterClusterCluster1 two three four 5 6 Cluster -1 0 1Position relative to the phosphorylated residueMembershipEnrichmentFIG. three. Dynamics on the rapamycin-regulated phosphoproteome. A, identification of drastically regulated phosphorylation web sites. The histogram shows the distribution of phosphorylation website SILAC ratios for 1h rapamycin/control (1h/ctrl) and also the distribution of unmodified peptide SILAC ratios (red). The cutoff for regulated phosphorylation web sites was determined based on two common deviations in the median for unmodified peptides.PMID:25046520 Unregulated internet sites are shown in black, and regulated web pages are shown in blue. The numbers of down-regulated and up-regulated phosphorylation web pages is indicated. B, the bar chart shows the distribution of phosphorylation internet sites into seven clusters, whereMolecular Cellular Proteomics 13.-7 -6 -5 -4 -3 -2 -1 0 1 two 3 four five 6494Phosphorylation and Ubiquitylation Dynamics in TOR Signalingbehavior employing a fuzzy c-means algorithm (Figs. 3B and 3C) (40, 48). Regulated phosphorylation web pages were clustered into six distinct profiles depending on the temporal behavior of those web sites. Distinct associations of GO terms inside each cluster (Fig. 3D and supplemental Figs. S2H 2M) indicated that phosphorylation websites with distinct temporal profiles had been involved in the regulation of different biological processes. Cluster 1 incorporated sites that showed decreased phosphorylation more than the time period of our experiment. This cluster included GO terms such as “signal transduction,” “ubiquitinprotein ligase activity,” and “positive regulation of gene expression” (supplem.